Details-enriched characterization of mobile populations. Morphological details from visuals can increase to the richness of flow cytometry knowledge. For example, the figure displays an or else conventional apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Every single populace to reveal morphologically distinct capabilities. These insights couldn't are received from multiplex staining alone.

Regardless of whether you’re a beginner or an expert, discover our resources to progress your analysis and know-how in flow cytometry.

Mix acoustic focusing technologies with significant-parameter Assessment for versatile spectral unmixing and standard workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing that has a superior-velocity brightfield digicam for simultaneous significant throughput flow cytometry and superior resolution brightfield imaging.

"A picture isn't very value one thousand dots, nonetheless it truly helps you to see photographs. It is excellent to view images that correlate with staining. As an example, AnnexinV-constructive cells which might be morphologically different than healthy cells, or CD14+ monocytes which have been greater and much more textured than CD3+/CD4+/CD14- lymphocytes.

Right here, a highly skilled person has gated singlets confidently. Immediately after assessing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate includes in excess of 4% aggregates.

Pair your Attune flow cytometer by having an autosampler for enhanced workflow performance, and add robotic integration for maximum throughput.

Consistent graphic high-quality even at higher throughput Acoustic concentrating in addition to a higher-velocity camera Blend to picture these T cells persistently at very low or superior flow charges. Effortlessly adjust concentrate and camera configurations to fulfill experimental necessities.

The Attune NxT cytometer would be the reputable workhorse instrument your lab can trust in for consistent and trustworthy performance. The Attune CytPix design incorporates a superior-velocity brightfield digicam and AI-driven image Investigation software package, with modern upgrades together with equipment Mastering applications and an intuitive consumer interface, enabling enhanced details precision, precision, and a lot quicker insights into sample biology.

End users can check out occasion visuals in serious-time to verify sample top quality, optimize protocols, and file the morphology of every cell populace identified for analysis. Imaging seize also Added benefits from acoustic focusing, which helps to placement the cells so that a pointy, centered image is received.

Seem waves help exact cell alignment, leading to Outstanding knowledge top quality and ~10X faster sample flow charges.

We Beforehand shown the power of imaging Vehicle-T/Ramos cell interactions. Permit’s take a look at just the populace of finest desire, the double optimistic events, to learn more. We could now use extended morphological parameters (below, circularity and skewness of intensity) to further look at the features of these populations and refine gating on these activities, expanding data robustness.

Crafted-in dimensionality reduction applications amplified resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to verify expected populace resolution in lysed entire human blood. Consumer can view situations from Every single cluster for visual confirmation of cell phenotypes.

Versatile payment system from Agilent Monetary Methods help you get the critical instrumentation you'll need for rapid job, or to mature your lab.

At the guts of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating technological innovation, which makes use of audio waves to meticulously align cells within the flow mobile. This progressive tactic surpasses common hydrodynamic approaches, resulting in more quickly processing speeds, Outstanding facts top quality, and greater sample throughput.

The get more info Attune Xenith cytometer can be a large-parameter spectral analyzer, providing both spectral unmixing and conventional flow abilities for comprehensive and multipurpose mobile Investigation.

Agilent Value Guarantee ensures you at the very least a decade of instrument use in the day you purchase. If not, We're going to credit you Together with the residual worth of the program towards an upgraded product.

What gave the impression to be a homogeneous populace is definitely much more diverse—an insight that needs to be deemed when interpreting effects.